Publications
NADf Chip, a Two-Color Microarray for Simultaneous Screening of Multigene Mutations Associated with Hearing Impairment in North African Mediterranean Countries.
Country: Middle East and North Africa
Classification: Hearing-Speech
Year of Publication: 2015
Study Type:
Quantitative Study
Sample Size:
151
The Research party:
1.The Processes Laboratory of Molecular and Cellular Screening, Center of Biotechnology of Sfax, University of Sfax
2. The Otorhinolaryngology Service, Habib Bourguiba University Hospital Sfax, Sfax, Tunisia
3.The Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Ahram Canadian University, Giza
4. The Department of Biochemistry and Molecular Biology,x Faculty of Pharmacy, British University in Egypt, Cairo, Egypt
Main Idea or Research Questions:
Hearing impairment (HI) is the most frequent sensory defect. HI is characterized by tremendous heterogeneity in both the genetic and clinical aspects. Therefore, the identification of the causal mutation is important for early diagnosis. To that end, explorers designed a cost-effective North African Deafness chip for rapid and simultaneous analysis of 58 mutations using multiplex PCR coupled with dual-color arrayed primer extension.. The North African Deafness chip allows for simultaneous genotyping of eight different samples, at a minimal cost and in a single day, and is therefore amenable to large-scale molecular screening of HI in North Africa.